Pair Name	Thymoquinone, Fluorouracil
Disease Info	[ICD-11: 2A00-2F9Z]	Solid tumour or cancer		Investigative
Gene Regulation	Down-regulation	Expression	COX2	hsa4513
	Down-regulation	Expression	CTNNB1	hsa1499
	Up-regulation	Expression	DKK1	hsa22943
	Up-regulation	Expression	GPX1	hsa2876
	Down-regulation	Expression	NOS2	hsa4843
	Down-regulation	Expression	CDH13	hsa1012
	Up-regulation	Expression	SMAD4	hsa4089
	Up-regulation	Expression	TGFB1	hsa7040
	Up-regulation	Expression	TGFBR2	hsa7048
	Down-regulation	Expression	VEGFA	hsa7422
	Down-regulation	Expression	WNT1	hsa7471
	Down-regulation	Expression	NFKB1	hsa4790
In Vivo Model	For induction of colorectal tumorigenesis, AOM was dissolved in normal saline and injected subcutaneously at a dose of 15 mg/kg, once weekly for 2 weeks.
Result	Our findings present the first report describing the in vivo enhancement effect of combined TQ and 5-FU against early stages of CRC; however, further studies are required to determine the value of this combination therapy in an advanced long-term model of CRC and also to realize its clinical potential.

Pair Name	Thymoquinone, Fluorouracil
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	CASP3	hsa836
	Up-regulation	Expression	CASP8	hsa841
	Up-regulation	Expression	CASP9	hsa842
	Down-regulation	Expression	CDK2	hsa1017
	Up-regulation	Expression	TP53	hsa7157
	Down-regulation	Expression	TYMS	hsa7298
In Vitro Model	BT-549	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_1092
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens (Human)	CVCL_0062
Result	TQ and 5-FU probably showed synergistic effect on both of cell cycle and apoptosis of tested TNBC cell lines. Our study reveals that TQ can synergise 5-FU action, and increase its anticancer efficiency against TNBC cells, which might be good choice in drug development for TNBC treatment.